Jumonji [Japanese for "cruciform/cross" also JARID2 (Jumonji/ARID domain-containing protein 2) and JMJ] is a 140-155kD member of the JMJ family of proteins.
It is a nuclear complex coordinator that interacts with the G9a and GLP methyltransferases to repress gene transcription.
Jumonji is expressed in mammalian embryonic neurons, megakaryocytes, hepatocytes and cardiomyocytes.
Human Jumonji is 1246aa in length.
It contains an NLS (aa1-131), a transcriptional repressor domain (aa132-222), a DNA binding region (aa558-709) and a nonfunctional histone demethylase domain (aa916-1042).
There are potential splice variants.
One shows an alternative start site at Met173, another shows a deletion of aa304-1151, and a third contains a 22aa substitution foraa1-60, coupled to a 50aa substitution for aa949-1246.
Over aa1-159, human Jumonji shares 96% aa identity with mouse Jumonji.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/mlWestern Blot: 0.1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human JARID2. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Purified, E. coli-derived, recombinant human Jumonji (rhJumonji; aa 1 - 159; Accession # Q92833).
