The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus.
With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers.
IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions.
Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members, have been identified.
In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have also been reported.
The functions of IL-17 RC, D, and E are not known.
Human IL-17B cDNA encodes a 180aa protein with a putative 20aa signal peptide.
Human and mouse IL-17B share 88% aa sequence identity.
Among IL-17 family members, IL-17B is most closely related to IL-17D, sharing 27% aa sequence homology.
IL-17B is expressed highly in spinal cord, and at lower levels in brain, kidney, lung, small intestine, prostate, testes, pancreas, adrenal gland and trachea.
Expression of IL-17B has also been detected in chondrocytes in articular cartilage.
IL-17B binds the IL-17B receptor but not IL-17 R and exhibits bioactivities distinct from those of IL-17.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human IL-17B. Species Crossreactivity: mouse. Cross-reactivity: rhIL-17, rhIL-17C, rhIL-17E, and rhIL-17F (<5%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：E. coli-derived recombinant human IL-17B, Gln21-Phe180 (Accession# Q9UHF5)
