IL-7 R-alpha (IL-7 receptor alpha; also CD127) is a 60-70kD member of the type I cytokine receptor family of molecules.
It is expressed on resting naïve and memory CD8+ T cells, DN thymocytes, pre-B cells, Th1 CD4+ T cells, dendritic cells and monocytes.
IL-7 R-alpha heterodimerizes with both the gc chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP receptor.
IL-7 R-alpha participates in T cell differentiation, naïve T cell survival, and CD4+ thymocyte proliferation.
Mature rat IL-7 R-alpha is a 437aa type I transmembrane glycoprotein.
It contains a 219aa extracellular region (aa21-239) that possesses one type III fibronectin domain (aa129-222), and a 193aa cytoplasmic tail that shows a key phosphorylation site at Tyr447.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes rat IL-7 R-alpha.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Purified, NS0-derived, recombinant rat IL-7 R alpha, extracellular domain.
