84-1174-09 Insulin-Like Growth Factor Binding Protein-Related Protein 10 (IGFBP-rP10, Kazal-type Serine Protease Inhibitor Domain-containing Protein 1, Bone and Odontoblast-expressed Protein 1, Insulin-like Growth Factor-binding-related Protein 10, IGFBP-rP10, IGFBP-related Protein 10, Insulin-like Growth Factor-binding-related Protein 4, Kazald1, Bono1) Biotin 50ug I7661-17W1
84-1174-09 Insulin-Like Growth Factor Binding Protein-Related Protein 10 (IGFBP-rP10, Kazal-type Serine Protease Inhibitor Domain-containing Protein 1, Bone and Odontoblast-expressed Protein 1, Insulin-like Growth Factor-binding-related Protein 10, IGFBP-rP10, IGFBP-related Protein 10, Insulin-like Growth Factor-binding-related Protein 4, Kazald1, Bono1) Biotin 50ug I7661-17W1
IGFBP-rP10 (IGF binding protein-related protein 10; also Kazal-type serine protease inhibitor domain-contain protein 1 and BONO1) is a 37kD (predicted), secreted member of the IGFBP superfamily.
It is expressed by osteoblasts and odontoblasts, is associated with regions of mineralization, and stimulates the proliferation of osteoblasts in vitro.
Mature human IGFBP-rP10 is 274 aa in length.
It contains an IGF binding domain (aa49-124), a follistatin-like serine protease inhibitor region (aa125-168) and a C2-type Ig-like domain (aa172-269).
There is one splice variant that shows a 70 aa substitution for aa184-304.
Mature human IGFBP-rP10 is 87% aa identical to mouse IGFBP-rP10.
Applications:Western Blot: 0.1-0.2ug/mlOptimal dilutions to be determined by the researcher.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:50ug
Host:sheep
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.
Specificity:Recognizes human IGFBP-rP10. Species sequence homology: mouse (87%)
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Hu
Immunogen:Purified, NS0-derived, recombinant human IGFBP-rP10 (aa31-304; Accession # Q96I82).