ER alpha (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65-70kD member of the NR3 subfamily, nuclear hormone receptor family of proteins.
It is widely expressed, and serves as a strong activator of estrogen-responsive genes.
ER-a is normally quiescent and bound to heat-shock proteins and immunophilins.
Following b-estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ER-b, and binds to DNA with multiple co-activators.
Major phosphorylation sites exist at Tyr537, Ser167 and Ser118.
Phosphorylation at Ser118 regulates ER-alpha’s role in transcription and cell growth.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml with MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100nM estradiol for 60 minutesOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes endogenous human ER-alpha phosphorylated at S118.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding S118 of human ER alpha (Accession # P03372).
