EOMES (Eomesodermin; Eo from Greek meaning “dawn”/early in mesoderm; also TBR2) is a 72kD member of the TBR1 subfamily, T-box family of transcription factors.
It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes.
It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation.
Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm.
Here, it synchronizes a multicellular commitment to a cell lineage.
Human EOMES is 686aa in length.
It contains short poly-Ala, -Gly and -Asn motifs, and a DNA-binding T-box (aa276-456).
There is one isoform that shows a 19 aa insertion after Ser460.
Over aa471-686, human EOMES shares 91% aa identity with mouse EOMES.
Applications:Suitable for use in Western Blot.
Other applicationb s not tested.
Recommended Dilutions:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, 0.02% NaN3.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with PBS, 0.02% sodium azide.
Specificity：Recognizes human EOMES.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived recombinant protein corresponding to Gly471-Pro686 of human EOMES.
