CDSN (Corneodesmosin; also S protein) is a 52-56kD, secreted glycoprotein that has an unusually high content of Gly, Pro and Ser.
It is found in the desmosomes of spinous layer keratinocytes, and presumably uses its high Gly content to generate homophilic noncovalent intercellular bridges.
Mature human CDSN is 497aa in length.
It contains a Ser-rich region (aa62-464) and one Gly-rich domain (aa60-171).
CDSN undergoes sequential proteolytic processing to generate multiple fragments that vary in size from 46-43kD, to 35-30kD, to 15kD.
This sequential extracellular processing allows initially for CDSN incorporation into a functional adhesional junction, and later for the removal of adhesional glycine loops with a subsequent dissociation (desquamation) of cells.
There is one potential isoform termed the S protein that shows a two aa substitution for the C-terminal 29aa.
Over aa33-529, human CDSN shares 68% aa identity with mouse CDSN.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-0.2ug/ml.
The detection limit is ~2ng/lane under non-reducing and reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile TBS, 0.1% BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as lyophilized powder from PBS, 50ug BSA. Labeled with Biotin. Reconstitute with 250ul sterile Tris-buffered saline pH 7.3 (20mM Tris base,150mM sodium chloride), 0.1% BSA.
Specificity：Recognizes human CDSN.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Lys33-Pro529 (Ser153 deletion) of human Corneodesmosin, expressed in NS0 cells.
