CHD1 (Chromohelicase/ATPase DNA-binding protein 1) is a 200-205kD member of the SNF2/RAD54 helicase family of proteins.
It is an ATP-dependent chromatin remodeling factor that helps maintain chromatin in a transcriptionally active state.
In embryonic stem cells, CHD1 associates with the promoters of active genes, a condition that is associated with open chromatin and pluripotency.
Human CHD1 is 1710aa in length.
It contains two Ser-rich regions (aa1-70 and 117-137), two Chromo (chromatin-organizer-modifer) domains (aa272-364 and 389-452), a SNF2 family N-terminal domain (aa484-763) and a C-terminal helicase domain (aa792-943).
There are at least 30 Ser/Thr phosphorylation sites.
Over aa1531-1710, human CHD1 shares 92% aa identity with mouse CHD1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human CHD1. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Purified, E. coli-derived, recombinant human CHD1 (Lys1531-Thr1710; Accession # O14646).
