Caveolin-1 is a palmitoylated 22kD membrane-associated protein in caveolae which are cholesterol-rich invaginations in the plasma membrane involved in vesicular transport and the regulation of lipid rafts and signal transduction.
Caveolin-1 expression is dysregulated during cancer progression and exhibits both positive and negative effects on tumor progression.
The central region of Caveolin-1 (aa105-125) is buried in the lipid layer, while the N- and C-terminal flanking regions are exposed to the cytoplasm and interact with many other proteins.
Alternate splicing in human, mouse, and rat Caveolin-1 generates an isoform with a deletion of the N-terminal 31 residues.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, 0.02% sodium azide.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS, 0.02% sodium chloride.
Specificity：Recognizes human Caveolin-1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：E.coli-derived recombinant human Caveolin-1 (rhCaveolin-1; aa1-104; Accession # Q03135).
