Caspase-1, also known as IL-1b-converting enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis.
The active enzyme consists of a complex of two 20kD (aa120-297) and two 10kD (aa317-404) subunits which associate following cleavage of inactive precursors.
Caspase-1 is required for proteolytic cleavage of the IL-1b precursor to form the active proinflammatory cytokine.
Alternate splicing generates several additional Caspase-1 isoforms with deletions in the propeptide regions or also in the mature subunits.
Within the large subunit, human Caspase-1 shares 61% aa sequence identity with mouse and rat Caspase-1.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.25ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, 0.02% sodium azide.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with PBS, 0.02% sodium chloride.
Specificity：Recognizes human Caspase-1. Species sequence homology: mouse, rat (61%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：E.coli-derived recombinant human Caspase-1 (aa120-297).
