84-1173-86　BOK (Bcl-2-related Ovarian Killer Protein, hBOK, Bcl-2-like Protein 9, Bcl2-L-9, BCL2L9) 100ug　B2530-01A

特徴

• BOK (Bcl-2-related ovarian killer protein; also Bcl2L9 and Mtd) is a 27kD member of the Bax subgroup of the Bcl-2 family of molecules.
• It is expressed in spermatocytes, ovarian granulosa cells and liver cells.
• BOK is activated by E2F proteins, and sensitizes replicating cells to apoptosis when under stress.
• It is normally found in the cytosol, and upon activation, becomes incorporated into mitochondrial membranes.
• Human BOK is 212aa in length and contains one BH4 domain (aa32-44), one BH3 domain (aa66-82), a BH1 domain (aa112-131) and a BH2 domain (aa164-178).
• A potential transmembrane span has been identified between aa189-210.
• BOK heterodimerizes with Mcl-1, BHRF-1, Bfl-1 and Bnip3, and is known to form heterooligomers.
• There are two potential splice variants.
• One contains an alternative start site at Met145, while another shows a five aa substitution for aa117-212.
• Over aa15-123, human BOK shares 94% aa identity with mouse BOK.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS, 0.02% sodium azide.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.