AR (Androgen receptor) is a 99kD (predicted) member of the NR3 subfamily, nuclear hormone receptor family of proteins.
Due to a high number of Gln and Pro residues, it runs anomalously at 100-120kD in SDS-Page.
It is widely expressed, being found in neurons, endothelial cells, osteoblasts, chrondrocytes, mascrophages, adipocytes, and prostate epithelium.
Human AR is 919aa in length.
It contains three discrete domains: a “modulating” N-terminus (aa1-553) that is rich in Gln, Pro and Gly, a Zn-finger DNA-binding region (aa554-635), and a ligand-binding domain (aa672-917).
AR is highly polymorphic at the N-terminus, with total Gln and Gly residues differing by seven or more residues among individuals.
Multiple potential splice forms exist, including an alternative start site at Met189 and a sevenaa substitution foraa1-538 that generates a 45kD isoform.
AR does homodimerize, apparently with multiple isotypes.
Overaa661-920, human and mouse are identical inaa sequence.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/mlELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human Androgen Receptor.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Purified, E. coli-derived, recombinant human Androgen Receptor (aa660-919; Accession # P10275).
