Aldehyde dehydrogenase 1A1 (ALDH1A1), also known as retinal dehydrogenase 1, is the second enzyme of the major oxidative pathway of alcohol metabolism.
Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations.
ALDH1A1 also belongs to the group of corneal crystallins that help maintain the transparency of the cornea.
(Retinal + NAD+ + H2O = retinoate + NADH)
Applications:Suitable for use in ELISA, Western Blot, and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/mlELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity:Recognizes human Aldehyde Dehydrogenase 1-A1.
Isotype:IgG
Calc Applications Abbrev:E IP WB
Calc Crossreactivity:Hu
Immunogen:E. coli-derived recombinant protein correponding to Ser2-Ser501 of human Aldehyde Dehydrogenase 1-A1/ALDH1A1.