84-1173-61　Tumor Necrosis Factor Receptor 1 (TNFR1, Tumor Necrosis Factor Receptor Superfamily Member 1A, TNF-R1, Tumor Necrosis Factor Receptor Type I, TNF-RI, TNFR-I, p55, p60, CD120a, TNFRSF1A, TNFAR) 100ug　T9161-07K

特徴

• Tumor necrosis factor receptor 1 (TNFR1), a potent cytokine, elicits a broad spectrum of biologic responses which are mediated by binding to a cell surface receptor.
• Its gene is located on 12p13.
• The coding region and the 3-prime untranslated region of TNFR1 are distributed over 10 exons.
• There are 2 different proteins that serve as major receptors for TNF-alpha, one associated with myeloid cells and one associated with epithelial cells.
• Additionally, TNFR1 associates with the MADD protein through a death domain-death domain interaction.
• MADD provides a physical link between TNFR1 and the induction of mitogen-activated protein (MAP) kinase (e.g., ERK2) activation and arachidonic acid release.
• TNFR1-induced apoptosis involves 2 sequential signaling complexes.
• Complex I, the initial plasma membrane-bound complex, consists of TNFR1, the adaptor TRADD, the kinase RIP1, and TRAF2 and rapidly signals activation of NF-kappa-B.
• In a second step, TRADD and RIP1 associate with FADD and caspase-8, forming a cytoplasmic complex, complex II.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.1-0.2ug/ml Immunohistochemistry (Paraffin): 0.5-1ug/ml.
• Antigen retrieval by heat is recommended.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.