84-1173-59　Tumor Necrosis Factor alpha (TNFa, Tumor Necrosis Factor, Cachectin, TNF-alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, TNF, TNFSF2) 100ug　T9160-29J

特徴

• Tumor necrosis factor-alpha (TNFA) also kowns as TNFR1, It is a potent cytokine, elicits a broad spectrum of biologic responses which are mediated by binding to a cell surface receptor.
• There are 2 different proteins that serve as major receptors for TNF-alpha, one associated with myeloid cells and one associated with epithelial cells.
• TNFR1 maps to 12p13.
• TNFR1 signaling is also known to activate the transcription factor NF-kappa B and promote survival.
• Tumor necrosis factor, alpha-induced protein 1 (endothelial), also known as TNFAIP1, is a human gene.The gene, present in single copy, was located in the 17q22-q23 region.
• This gene was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells.
• Studies of a similar gene in mouse suggest that the expression of this gene is developmentally regulated in a tissue-specific manner.
• The protein is involved in the primary response of the endothelium to TNF.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1ug/ml.
• The detection limit is ~2.5ng/lane under non-reducing and reducing conditions
• Immunohistochemistry (Paraffin): 1ug/ml Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.