Trk A (Neurotrophic tyrosine kinase receptor A) is the high affinity catalytic receptor for the neurotrophin,Nerve Growth Factor (NGF).
Higher affinity binding of NGFR can achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA(NTRK1), TRKB (NTRK2), and TRKC (NTRK3).
TRKA, TRKB, and TRKC are specific for or 'preferred by' NGF, NTF4(Neurotrophin-4) and BDNF, and NTF3(Neurotrophin-3), respectively.
NTF3 also binds to TRKA and TRKB, but with significantly lower affinity.
The absence of TrkA(NGFR) expression was associated with a strong increase in the Sp3 repressor short isoform(s) and a lack of the Sp3 activator long isoform.
Sp3 is a bifunctional transcription factor that has been reported to stimulate or repress the transcription of numerous genes.1 Indo et al.
(1996) concluded that defects in TRKA cause CIPA (Congenital insensitivity to pain with anhidrosis ) and that the NGF-TRKA system has a crucial role in the development and function of the nociceptive reception as well as establishment of thermoregulation via sweating in humans.
These results also implicate genes encoding other TRK and neurotrophin family members as candidates for developmental defect(s) of the nervous system.
Applications:Suitable for use in Western Blot..
Other applications not tested.
Recommended Dilution:Western Blot:1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH20.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Specificity：Recognizes human Trk-A. Species Cross-Reactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to a sequence at the middle region of human Trk-A, identical to the related rat and mouse sequence.
