Terminal Deoxynucleotidyl Transferase, also known as TdT and terminal transferase, is a unique DNA polymerase without template direction catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers.
Its gene is mapped to the region 10q23-q24.2 And TDT Cdna contains an open reading frame of 1,530 base pairs corresponding to a protein containing 510aa.
TDT may be responsible for inserting nucleotides (N regions) at the V(H)-D and D-J(H) junctions of immunoglobulin genes.
The enzyme is present in immature thymocytes, some bone marrow cells,transformed pre-B and pre-T cell lines, and leukemia cells.
Additionally, TdT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule.
Unlike most DNA polymerases it does not require a template.
The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends.
Cobalt is a necessary cofactor.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot:1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH20.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Specificity：Recognizes human TDT. Species Sequence Homology: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to a sequence at the C-terminal of human TDT, identical to the related rat and mouse sequence.
