84-1173-21 STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5, STAT5, Signal Transducer and Activator of Transcription 5A, STAT5A, STAT5B, Signal Transducer And Activator Of Transcription 5B) 50ug S7972-07N2
84-1173-21 STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5, STAT5, Signal Transducer and Activator of Transcription 5A, STAT5A, STAT5B, Signal Transducer And Activator Of Transcription 5B) 50ug S7972-07N2
STATs, such as STAT5, are proteins that serve the dual function of signal transducers and activators of transcription in cells exposed to signaling polypeptides.
In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.
STAT5 is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones.
STAT5A and STAT5B knockout mice show important roles in prolactin-mediated mammary gland development and growth hormone-mediated induction of sexual dimorphism, respectively.
Abnormal activity of certain STAT family members, particularly STAT3 and STAT5, is associated with a wide variety of human malignancies, including hematologic, breast, head and neck, and prostate cancers.
STAT5A and STAT6, are selectively activated when the heart is subjected to ischemic injury, whereas activation of STAT3 and STAT5A is involved in myocardial hypertrophy.
STAT5 and STAT3, as these two STAT molecules are required for normal breast development and involution, respectively, and may play an important role in breast carcinogenesis.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot:1:500-1:1,000Immunohistochemistry:1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human STAT5 when phosphorylated at tyrosine 694. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to human STAT5 around the phosphorylation site of tyrosine 694 (D-G-YP-V-K).
