Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors.
Tyrosine 418 is located in the catalytic SH2 domain and is one of the autophosphorylation sites.
Full catalytic activity of Src requires phosphorylation of tyrosine 418.
This activity is negatively regulated when tyrosine 529 of Src is phosphorylated by Csk.
This conformation holds Src in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot:1:500-1:1,000Immunohistochemistry:1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Scr when phosphorylated at Tyr418. Species Cross-Reactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to human Scr around the phosphorylation site of tyrosine 418 (N-E-YP-T-A).
