84-1172-79　［取扱停止］Shc, phosphorylated (Tyr427) (SHC-transforming Protein 1, SHC-transforming Protein 3, SHC-transforming Protein A, Src Homology 2 Domain-containing-transforming Protein C1, SH2 Domain Protein C1, SHC1, SHCA) 50ug　S1010-90L

特徴

• Shc possesses SH2 and PTB domains.
• It serves a scaffolding function in signaling for a variety of receptor tyrosine kinases.
• Shc exists in p46, p52 and p66 isoforms.
• These isoforms are produced by using alternative translation initiation sites or a differentially spliced message.
• In response to extracellular signals, the SH2 and PTB domains of Shc interact with the activated receptors, leading to phosphorylation of Shc on three different tyrosine residues: Tyr239, Tyr240 and Tyr317.
• Grb2/Sos binds to Shc phosphorylated at these sites, activating the Ras/Raf/MAPK pathway.
• Both Shc expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T cell development.
• Applications:Suitable for use in Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot:1:500-1:1,000Immunohistochemistry:1:50-1:100 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.