90kD ribosomal S6 kinases (RSK1-3) are a family of broadly expressed serine/threonine kinases activated by extracellular signal-regulated protein kinases (Erk1 and 2) in response to many growth factors, polypeptide hormones and neurotransmitters.
p90RSK is activated by MAPK in vitro and in vivo via phosphorylation.
Several sites, such as Ser380, Thr359 and Ser363, are important for its activation.
RSK3 resembles p90RSK by having two kinase domains connected by a regulator linker region.
Phosphorylation of p90RSK (and likely RSK2 and RSK3) in the linker region is critical for its activity.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot:1:500-1:1,000 Immunohistochemistry:1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse Rsk, 90kD when phosphorylated at threonine 348. Species Cross-Reactivity: human, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to mouse p90RSK around the phosphorylation site of threonine 348 (S-R-TP-P-R).
