RPS6KB1 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases.
This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein.
The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation.
Amplification of the region of DNA encoding the gene for RPS6KB1 and overexpression of this kinase are seen in some breast cancer cell lines.
Applications:Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence:1:100-1:200 Western Blot:1:500-1:1,000 Immunohistochemistry:1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human RPS6KB1 when phosphorylated at serine 424. Species Cross-Reactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to human RPS6KB1 around the phosphorylation site of serine 424 (P-V-SP-P-V).
