DNA repair protein RAD51 homolog 1, also known as RAD51A, is a human gene.
The Rad51 gene,HsRAD51, is a homolog of RecA of Escherichia coli and functions in recombination and DNA repair.
BRCA1 and BRCA2 proteins form a complex with Rad51, and these genes are thought to participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair.
RAD51 is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage.
BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein.
Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot:1ug/ml Immunohistochemistry (paraffin):1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH20.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Specificity：Recognizes human RAD51A. Species Cross-Reactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to a sequence at the N-terminal of human RAD51A, different from the related rat sequence by 1 aa.
