PYK2 is involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway.
PKY2 may represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity.
Interacts with the SH2 domain of Grb2.
May phosphorylate the voltage-gated potassium channel protein Kv1.2.
PYK2 activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity.
It's been shown to be involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1,000 Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Pyk2 when phosphorylated at tyrosine 402. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to human Pyk2 around the phosphorylation site of tyrosine 402 (D-I-YP-A-E).
