84-1172-32　［取扱停止］Protein Kinase C, theta (Protein Kinase C theta Type, nPKC-theta, PRKCQ, PRKCT) 50ug　P9103-68F

特徴

• Protein Kinase Ct (PKCt) is an 80kD member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and homeostasis.
• Transgenic mice over-expressing dominant negative PKCt develop hyperinsulinimia.
• PKCt is involved in JNK activation and also plays a specialized role in TCRmediated activation of T and B cells.
• The activation of PKCt in T cells is associated with its recruitment to the membrane and is mediated by PI3-kinase and Vav.
• PKCt is a constitutively competent kinase and is auto-phosphorylated on serine 676 in the turn loop and serine 695 in the hydrophobic loop.
• The phosphorylation on serine 695 contributes to the catalytic activity of PKCt.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1,000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.