Protein kinase C (PKC) mediates a wide range of biological responses, including serotonin release from platelets, histamine release from mast cells, glycogen hydrolysis in the liver, fat synthesis by adipocytes, and many others.
PKC was described as a serine/threonine protein kinase activated by the presence of Ca2+, phospholipids, and sn-1,2 diacylglycerol (Nishizuka, 1986).
In humans, PKC is found as 11 different isoforms which vary in amino acid sequence, structure, subcellular localization, tissue distribution, substrate specificity, and responses to extracellular signals.
The PKC isoforms can be grouped based on their requirements for Ca2+ and phospholipids for activation.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1,000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Protein Kinase C, beta 1. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic non-phosphopeptide corresponding to human PKC beta around the phosphorylation site of threonine 641 (E-L-TP-P-T).
