Placental growth factor (PGF, also known as PLGF) codes for an angiogenic factor expressed in placental tissue that is similar to vascular permeability factor/vascular endothelial growth factor (VPF/VEGF).
It is a glycosylated dimeric secreted protein able to stimulate endothelial cell growth in vitro.
PGF is located on chromosome 14 and has been conserved in evolution.
It belongs to the family of vascular endothelial growth factors (VEGFs) and binds to the flt-1 VEGF receptor.
PLGF-2, which is a PLGF isoform, binds neuropilin-1 and 2 in a heparin-dependent manner.
PGF regulates inter- and intra molecular cross talk between the VEGF RTKs Flt1 and Flk1 and stimulates the phosphorylation of specific Flt1 tyrosine residues and the expression of distinct downstream target genes.
Moreover, it plays an important role in pathological angiogenic events and with exerting its biological activities through binding to VEGFR1.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-2ug/mlImmunohistochemistry (Paraffin): 1-2ug/ml in formalin fixed and paraffin embedded tissuesImmunohistochemistry (Frozen): 2ug/ml using formalin or acetone fixed tissuesOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 50% glycerol. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human PLGF. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to a sequence mapping near the N-terminal of human PLGF, different from the related rat sequence by two different amino acids.
