Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water soluble phosphorylated derivatives of the lipid head groups.
A number of these enzymes have specificity for phosphoinositides.
Of the phosphoinositide specific phospholipase C enzymes, C beta is regulated by heterotrimeric G protein coupled receptors, while the closely related C gamma 1 and C gamma 2 enzymes are controlled by receptor tyrosine kinases.
The C gamma 1 and C gamma 2 enzymes are composed of phospholipase domains that flank regions of homology to noncatalytic domains of the SRC oncogene product, SH2 and SH3.
Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Phospholipase C, gamma 2. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic non-phosphopeptide corresponding to human PLC-gamma2 around the phosphorylation site of tyrosine 1217 (F-L-YP-D-T).
