p73 was identified as a long-lost analog of tumor suppressor protein, p53.
p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells.
Significant homology between p53, p63, and p73 (approximately 63% aa identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression.
The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections.
Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line.
These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy-terminal region of p73 may be important for the functions of this protein.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot:1:500-1:1,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human p73 when phosphorylated at tyrosine 99. Species Cross-Reactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to human p73 around the phosphorylation site of tyrosine 99 (S-P-YP-A-Q).
