Cyclin-dependent kinase inhibitor 1B (CDKN1B), also known as p27 (KIP1), is a cyclin-dependent kinase (Cdk) inhibitor implicated in G1 phase arrest, which negatively regulates G1 phase progression in response to TGF beta and represents a tumor suppressor gene.
Human p27 gene is mapped to chromosome 12p12.3 p27 can be both an inhibitor and a substrate of cyclin E-CDK2.
p27, abundant in quiescent cells and drops after serum stimulation, plays a role in mediating VSMC hypertrophy.
p27 degradation is subject to dual control by the accumulation of both SKP2 and cyclins following mitogenic stimulation.
It regulates cell proliferation by binding to and modulating the activity of cyclin-dependent kinases.
Reduced p27 activity is fundamental for the development of many human malignancies including breast, prostate, colon and gastric carcinomas.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot:1:500-1:1,000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human p27kip1 when hosphorylated at threonine 187. Species Cross-Reactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to human p27Kip1 around the phosphorylation site of threonine 187 (E-Q-TP-P-K).
