84-1171-71　Orexin Receptor 100ug　O7503-01

特徴

• Orexins are endogeneous peptides that activate Orexin Receptor type 1 and type 2, two closely related G-protein coupled receptors.
• Orexins-A and -B are hypothalamic peptides derived from a precursor called preproorexin and affect the stimulation of food intake.
• They act on G protein receptors named orexin receptor-1 (OX1R) and orexin receptor-2 (OX2R), respectively.
• Stimulation of these receptors by orexins causes an increase in intracellular calcium levels in hypothalamic cells in vitro.
• In vivo, orexins control feeding and may also be involved in modulating sleep-wake cycles and other hypothalamic functions.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 2ug/ml Immunohistochemistry (paraffin): 1-2ug/ml Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.