84-1171-60　［取扱停止］NOS III (Endothelial Nitric Oxide Synthase, eNOS, NOS 3) 50ug　N5350-26D

特徴

• Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system.
• It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium.
• Unlike typical neurotransmitters, NO is not stored in synaptic vesicle and does not act on membrane receptors.
• Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.
• NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-III, MW ~130-160kD).
• One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA).
• It is found in endothelium, adrenal glands, brain and platelets.
• The other principal group is inducible, Ca2+/calmodulin-independent, and inhibited by NMMA and L-NNA.
• It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells.
• Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II).
• Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible.
• Sequence homology among different cloned isoforms is ~ 50%.
• Human, rat, mouse and bovine eNOS/NOS-3 are ~1202-1205aa proteins.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot:1:500-1:1,000 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.