84-1171-48 Neuron Specific Enolase (NSE, Gamma-enolase, 2-phospho-D-glycerate Hydro-lyase, Neural Enolase, Enolase 2, ENO2) 100ug N2175-01L
特徴
- NSE (neuron specific enolase), also known as Enolase 2 (ENO2), is found in elevated concentrations in plasma in certain neoplasias.
- The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway.
- ENO2 gene contains 12 exons distributed over 9,213 nucleotides.
- Human neurone-specific enolase is mapped to chromosome 12p13.
- Applications:Suitable for use in Western Blot and Immunohistochemistry.
- Other applications not tested.
- Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 1-2ug/ml Heat-mediated antigen retrieval is recommended
- Optimal dilutions to be determined by the researcher.
- Storage and Stability:Lyophilized powder may be stored at -20°C.
- Stable for 12 months at -20°C.
- Reconstitute with sterile ddH2O.
- Aliquot to avoid repeated freezing and thawing.
- Store at -20°C.
- Reconstituted product is stable for 12 months at -20°C.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Further dilutions can be made in assay buffer.
仕様
- Size:100ug
- Host:rabbit
- Source Antibody:human
- Grade:Affinity Purified
- Purity:Purified by immunoaffinity chromatography.
- Form:Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
- Specificity:Recognizes human NSE. Species Crossreactivity: mouse, rat.
- Isotype:IgG
- Calc Applications Abbrev:IHC WB
- Calc Crossreactivity:Hu Mo Rt
- Immunogen:Synthetic peptide corresponding to aa 420-434, EARFAGHNFRNPSVL, from thr C-terminus of human NSE.
- Shelf Life:1year
- Swiss Prot Number:P09104
- EU Commodity Code:30021010
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