Human neurite outgrowth inhibitor (NOGO) cDNAs encodes 3 splice variants: NOGOA, NOGOB and NOGOC.
The longest cDNA, designated NOGOA, has an open reading frame of 1192aa.
It is a potent inhibitor of neurite growth and an IN-1 antigen produced by oligodendrocytes, and may allow the generation of new reagents to enhance CNS regeneration and plasticity.
Nogo-A, a member of the Reticulon family, is expressed by oligodendrocytes and associates primarily with the endoplasmic reticulum.
The acidic amino terminus of Nogo-A is detected at the cytosolic face of cellular membranes and may contribute to inhibition of axon regeneration at sites of oligodendrocyte injury.
A multivalent form of the N terminus of Nogo-A affects the morphology of both neurons and other cell types.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-0.5ug/ml Immunohistochemistry (Paraffin): 0.5-1ug/ml using HIEROptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human Neurite Outgrowth Inhibitor Protein A. Does not react with other proteins. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to a aa1170-1192, NKNVKDAMAKIQAKIPGLKRKAE at the C-terminal of human Neurite Outgrowth Inhibitor Protein A. The sequence is different in rat by two amino acids and by one amino acid in mouse.
