mTOR (Mammalian Target of Rapamycin, FRAP, RAPT or RAFT) is a large 289kD Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy.
mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity.
mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins.
These two multimeric protein complexes are designated mTORC1 and mTORC2.
mTORC1 consists of at least mTOR, Raptor, and G betaL.
mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis.
The other mTOR complex, mTORC2, is made up of at least mTOR, Rictor, G betaL, Sin1, Protor 1 and 2.
TORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473.
TORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC.
Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human mTOR when phosphorylated at serine 2448. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to human mTOR around the phosphorylation site of serine 2448 (T-D-SP-Y-S).
