MKK3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family.
This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade.
It phosphorylates and thus activates MAPK14/p38-MAPK.
This kinase can be activated by insulin, and is necessary for the expression of glucose transporter.
Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells.
The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis.
Applications:Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200Western Blot: 1:500-1:1,000Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MAP Kinase Kinase 3 when phosphorylated at serine 189. Species Crossreactivity: rat.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic phosphopeptide corresponding to human MKK3 around the phosphorylation site of serine 189 (V-D-SP-V-A).
