MAP kinase kinase (Mek1), also known as mitogen activated protein kinase kinase, MAP kinase kinase, ERK kinase 1 or microtubule-associated protein 2 kinase, is a 43kD dual-specificity protein kinase that phosphorylates threonine and tyrosine residues on MAP kinases such as Erk 1 and 2.
Phosphorylation by Mek1 increases Erksí ability to phosphorylate micrtubule-associated proteins and myelin basic proteins.
Mek1 is involved in diverse arrays of cellular processes such as stress-activated response, apoptosis, cytokine-induced cell proliferation, and DNA recombination during meiosis.
Mek1 is activated by phosphorylation on serine 218 and 222 by serine-threonine kinase, Raf-1, which is part of the p21ras signal transduction pathway.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1,000 Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1× TBS, 0.05% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid.
Specificity：Recognizes human MEK1. Species Crossreactivity: mouse, monkey.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mk Mo
Immunogen：Synthetic peptide (KLH-coupled) corresponding to human MEK1.
