The matrix metalloproteinase 16(MMP16) protein consists of 604aa and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q211.
Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14 , MMP15 , and MMP172.
Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms.
MMP16 is expressed as a 12-kb transcript in brain, placenta, heart, and some carcinoma cell lines, but is not detectably expressed in lung, kidney, liver, spleen, and muscle.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (frozen, paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Specificity：Recognizes human MMP16. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide mapping at the C-terminal of human MMP16, identical to the related rat sequence.
