DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase.
It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML.
DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs.
DOK2 binds p120 (RasGAP) from CML cells.
It has been postulated to play a role in mitogenic signaling.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot:1:500-1:1,000 Immunohistochemistry:1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Dok1 when phosphorylated at tyrosine 398. Species Cross-Reactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to human Dok1 around the phosphorylation site of tyrosine 398 (E-G-YP-E-L).
