DARPP-32 is a dopamine (DA) and cAMP-regulated 32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998).
The protein inhibits protein phosphatase I when it is phosphorylated on Thr34.
In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999).
Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission.
In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).
Applications:Suitable for use in Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human DARPP32 when phosphorylated at Thr75. Species Cross-Reactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IF
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to human DARPP-32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P).
