84-1168-80　Dab1 (Disabled Homolog 1, DAB1) 50ug　D0900-15B

特徴

• Disabled 1 (Dab1) is an 80kD protein that is encoded by the Disabled-1 gene locus which is mutated in scrambler and yotari mutant mice.
• Phenotypically, the mutation of this gene produces motor defects and ataxia, disruption of neuronal migration and severe cerebellar hypoplasia.
• Dab1 is an intracellular adapter protein that functions in downstream signaling events initiated by the secreted protein reelin.
• Dab1 contains a phosphotyrosine binding (PTB) domain in the amino terminus.
• Tyrosine phosphorylation of Dab1 is increased by reelin binding to the Very Low Density Lipoprotein Receptor (VLDLR) and Apolipoprotein E Receptor 2 (ApoER2) through stimulation of Src family kinases (e.g., Fyn).
• Src family kinase and c-Abl activities are themselves then stimulated by binding to tyrosine phosphorylated Dab1.
• Dab1 also mediates activation of Akt (PKB) by reelin resulting in inhibition of glycogen synthase kinase 3b (GSK-3b) and decreased phosphorylation of the microtubule-associated protein, Tau.
• Dab1 serine 491 is phosphorylated in a Cdk5-dependent manner and regulates, likely indirectly, Reelin-induced signaling during neural cortex development.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.