Cytokeratin 8 (CK8), also known as Keratin 8 (K8), is a type II keratin.
Among the various intermediate filament (IF) proteins, cytokeratin 8 (CK8) is especially remarkable as it is produced early in embryogenesis, is the only type-II CK occurring in many simple epithelial cells, and can also be synthesized in certain non-epithelial cells.
Cytokeratin 8 mRNA was abundant in fetal small intestine, placenta, pancreas, lung, liver, and kidney.
Levels of cytokeratin 8 mRNA in placenta increased slightly during pregnancy.
He et al.
(2002) presented evidence that K8 is a new cytoplasmic target for JNK in Fas receptor-mediated signaling.
The functional significance of this phosphorylation could relate to regulation of JNK signaling and/or regulation of keratin dynamics.
Applications:Suitable for use in Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Keratin 8 when phosphorylated at serine 74.
Isotype：IgG
Calc Applications Abbrev：IF
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to human Keratin 8 around the phosphorylation site of serine 74 (L-L-SP-P-L).
