In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr.
This maintains the inactive state until the end of G2-phase.
The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary.
This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded.
This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis.
So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Ab-2 is excellent for biochemical applications ans co-precipitates cyclin B1 associated p34cdc2.
Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200Immunohistochemistry: 1:50-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Cyclin B1 when phosphorylated at serine 147.
Isotype：IgG
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to human Cyclin B1 around the phosphorylation site of Serine 147 (A-F-SP-D-V).
