84-1168-68　［取扱停止］CrkL (Crk-like Protein, CRKL) 50ug　C7925-50E

特徴

• CrkL is a 39kD adaptor protein, originally cloned in proximity to the BCR gene on chromosome 22, which has a key regulatory role in hematopoietic cells.
• CrkL has one SH2 and two SH3 domains, with 60% homology to CrkII.
• Structurally, the amino-terminal SH3 domain of CrkL has been shown to bind proteins such as C3G, SOS, PI3K, c-Abl or BCR/Abl.
• The SH2 domain of CrkL can bind to tyrosine phosphorylated proteins such as Cbl, HEF1, CAS or paxillin.
• CrkL is involved in various signalings initiated by different cytokines and growth factors.
• The biological outcomes of the Crk-activated signal transduction cascades include the modulation of cell adhesion, cell migration, immune cell responses and so on.
• CrkL is a prominent substrate of the BCR/Abl oncoprotein in chronic myelogenous leukemia and binds to both BCR/Abl and c-Abl.
• CrkL is prominently and constitutively tyrosine phosphorylated in CML neutrophils and is not phosphorylated in normal neutrophils.
• Moreover, stimulation of normal neutrophils with cytokines and agonists does not induce tyrosine phosphorylation of this protein, indicating that it may be a useful target for therapeutic intervention or as a disease marker.
• Tyrosine 207 in CrkL is the BCR/Abl phosphorylation site.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA:1:20,000 Western Blot:1:500-1:1,000Immunohistochemistry:1:50-1:100 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.