Cyclooxygenase 1 (COX1), also known as Prostaglandin-endoperoxide synthase (PTGS1), is the key enzyme in prostaglandin biosynthesis.
The gene was approximately 40kb long, with 11 protein-coding exons.
There were 599aa residues with a calculated molecular mass of approximately 68kD.
By analysis of a human/hamster somatic hybrid DNA panel, Funk et al.
(1991) demonstrated that the PTGS1 gene maps to chromosome 9.
Human prostaglandin endoperoxide synthase exhibited 91% aa identity with the sheep enzyme.
Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot (Human, mouse, rat): 0.1-0.5ug/mlImmunohistochemistry (Paraffin) (Human): 0.5-1ug/ml.
Antigen retrieval using heat is recommended
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human COX 1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa35-52, CCYYPCQHQGICVRFGLD near the N-terminal of human COX1, different to the related rat and mouse sequence by 2aa.
