84-1168-59　Corticotrophin Releasing Factor (Corticotropin-releasing Factor, CRF, Corticoliberin, Corticotropin Releasing Hormone, CRH) 100ug　C7903-90B

特徴

• CRH is a 41aa peptide derived by enzymatic cleavage from a 191aa preprohormone and is located on 8q13.
• It is made not only in the hypothalamus but also in peripheral tissues, such as T lymphocytes, and is expressed in very large amounts in the human placenta.
• As a placental clock, CRH controls the duration of pregnancy and that the timing of the rise in CRH may permit prediction of the onset of labor.
• Furthermore, CRH is the most proximal element of the HPA axis, and it acts as a central coordinator for neuroendocrine and behavioral responses to stress.
• In addition, placental secretion of CRH is a marker of the 'placental clock' that is active from an early stage in human pregnancy and determines the length of gestation and the timing of parturition and delivery.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1ug/mlImmunohistochemistry (Frozen, paraffin): 0.5-1ug/ml to detect CRH in formlin fixed, paraffin embedded tissues.
• Boiling the sections is required
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.