84-1168-48　CKTSF1B1 (Gremlin1, DRM, Cysteine knot superfamily 1 BMP antagonist 1, Increased in high glucose protein 2, IHG-2, Down-regulated in Mos-transformed cells protein, DAN domain family member 2, Cell proliferation-inducing gene 2 protein GREM1, CKTSF1B1, DAND2) 100ug　C5818-06

特徴

• Gremlin-1 is a secreted cytokine that may play a role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop.
• It down-regulates the BMP4 signaling in a dose-dependent manner.
• It also acts as inhibitor of monocyte chemotaxis.
• It is highly expressed in small intestine, fetal brain and colon.
• Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle.
• In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus.
• Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts.
• Gremlin-1 is induced through TGF-beta 1-mediated pathways in mesangial cell and it is down-regulated in tumor cell lines.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot (Human, rat): 1ug/ml Immunohistochemistry (Paraffin) (Human, rabbit): 1ug/ml Antigen retrieval needed
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.