84-1168-15　［取扱停止］CDC2, phosphorylated (Tyr15) (Cell Division Protein Kinase 1, Cell Division Control Protein 2 Homolog, Cyclin-dependent Kinase 1, CDK1, p34 Protein Kinase, CDK1) 50ug　C2549-49Y

特徴

• Entry of all eukaryotic cells into M-phase of the cell cycle is regulated by activation of cdc2 kinase.
• Activation of cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161.
• The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of tyrosine 15 and threonine 14.
• Phosphorylation at Tyr15 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases.
• Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase.
• cdc2 activation requires phosphorylation at Thr161, which is attributed to the cdk-activating kinase CAK, a complex of cdk7 and cyclin H.
• Phosphorylation at Thr161 is also required for association with cyclin A.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot:1:500-1:1,000 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.