Caspase 2 is a member of ICH1 group of cystein proteases involved in the activation of apoptotic pathway.
It is synthesized as a procaspase2 containing a prodomain, a large subunit and a small subunit.
RAIDD, another molecule involved in triggering the apoptotic pathway recruits caspase 2 through its CARD domain.
Procaspase 2 iscleaved by active caspases 1 and 3 and granzyme into a heterodimer of large and small subunit, both of which are required for the activity.
Caspase 2 exists in two isoforms, long and short created through the alternate splicing of the mRNA.
The long form activates apoptosis while, the short form is inhibitory.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlImmunohistochemistry (paraffin): 1-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Specificity：Recognizes human Caspase 2. Species Cross-Reactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide mapping at the C-terminal of human Caspase 2, identical to the related rat sequence.
