Three catenin proteins, a, b and y, bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin.
The catenin/cadherin complexes play an important role mediating cellular adhesion.
Not only associated with E-cadherin, a-catenin was also shown to associate with other members of the cadherin family, such as Ncadherin and P-cadherin.
b-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule.
b-catenin has also been found in complexes with the tumor suppressor protein APC.
y-catenin, also known as plakoglobin, is a protein that binds with a-catenin and N-cadherin.
It has been shown that the transmembrane phosphatase PTP associates with catenin/cadherin complexes and may regulate complex signaling.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot:1:500-1:1,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Catenin alpha1. Species Cross-Reactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic non-phosphopeptide corresponding to human a-catenin around the phosphorylation site of serine 641 (D-D-SP-D-F).
